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Innovative application of helium-neon laser: enhancing the germination of Adansonia digitata and evaluating the hepatoprotective activities in mice

机译:氦氖激光的创新应用:增强患有adansonia digitata的萌发并评估小鼠的肝脏保护活性

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摘要

The laser pretreatment of seed is drawing pronounced attention from the scientific community for its positive impact in boosting germination, , and growth of plants. In this study, the laser pretreatment of Adansonia digitata (A. digitata) seeds was evaluated. Eight laser treatments were conducted at different powers, 10, 20, 40, and 80 mW, with the two-time interval for each power at 2 and 4 min. The outcomes indicated that the most efficient irradiation was 10 mW/2 min which induces the highest germination rate and polyphenolic contents for seeds. Based on these results, the animal experimental design was processed to assess the hepatoprotective activity of A. digitata extracts obtained through the optimum laser preillumination to enhance the resistance of liver damage in mice. The total phenol and flavonoid contents and the antioxidant properties of the methanolic extracts were estimated in vitro. The CCl4 was used to induce hepatotoxicity in mice. The animals were divided into five groups. The sera of the treated animals were used for the determination of transaminases, and the liver homogenates were used for the determination of antioxidant status, and further liver tissues were subjected to verify the anti-apoptotic effect of A. digitata methanolic extract. The in vivo results showed that the methanolic extract exposed to laser treatment at 10 mW/2 min provided better hepatoprotective capacity than the other treatments. Administration of A. digitata extract not only offered a significant decrease in liver enzyme activity but also markedly improved the antioxidant status and reduced the apoptotic progression induced by CCl4 toxicity in liver tissue.
机译:种子的激光预处理是从科学界汲取的关注,以积极影响促进萌发,以及植物的生长。在这项研究中,评估了Adansonia digitata(A. Digitata)种子的激光预处理。在不同的功率,10,20,40和80mW处进行八种激光处理,每次电力为2和4分钟。结果表明,最有效的辐射是10mW / 2分钟,诱导种子的最高萌发率和多酚含量。基于这些结果,处理动物实验设计以评估通过最佳激光溢出物获得的A. Digitata提取物的肝保护活性,以增强小鼠肝损伤的抗性。估计总苯酚和类黄酮含量和甲醇醇提取物的抗氧化性能。 CCL4用于诱导小鼠肝毒性。将动物分成五组。处理过的动物的血清用于测定转氨酶,并且肝匀浆用于测定抗氧化剂状态,并进行进一步的肝组织验证A. Digitata甲醇提取物的抗凋亡效应。体内结果表明,10mW / 2分钟暴露于激光处理的甲醇提取物提供比其他治疗更好的肝保护能力。 A. Digitata提取物不仅提供了肝酶活性的显着降低,而且显着改善了抗氧化剂状态并降低了CCL4毒性在肝组织中的凋亡进展。

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