A detailed study on the interaction of a novel water-soluble glycine bridged zinc(II) Schiff base coordination polymer with BSA: Synthesis, crystal structure, molecular docking and cytotoxicity effect against A549, Jurkat and Raji cell lines

摘要

A novel water-soluble glycine bridged zinc(II) Schiff base coordination polymer was synthesized by the condensation of 2,6-diformyl-4-methylphenol, glycine, and zinc(II) chloride. The complex was characterized by (HNMR)-H-1, FT-IR, elemental analyses and X-ray crystallography. The polymeric complex was built up of two glycine-bridged Zn/L moieties, where L denotes the Schiff base containing 2,6-diformyl-4-methylphenol and glycine in 1:1 M ratio. The carboxylic group of L was coordinated to the Zn atoms of the neighboring moieties; thus each Zn center was five-coordinated. The interaction between polymeric Zn(II) complex and bovine serum albumin (BSA) was studied by UV Vis, fluorescence, and synchronous fluorescence spectroscopic techniques. By considering the sign and values of the thermodynamic parameters (Delta H and Delta S), it is clear that the binding between BSA and complex was exothermic and entropy driven and electrostatic interactions between the complex and BSA was supposed. Site-selective binding studies revealed that the complex were mainly located in the region of site II (subdomain IIIA) in BSA. From the synchronous, fluorescence spectroscopic studies, it is concluded that complex could bind to tyrosine and tryptophan residues simultaneously. The K-b values indicated a high binding affinity of the complex to BSA. In vitro anticancer activity of the polymeric Zn(II) complex was evaluated against A549, Jurkat, and Raji cell lines by KTT assay. The complex was remarkably active against the cell lines and can be a good candidate for an anticancer therapy. Theoretical docking studies were performed to further investigate the BSA binding interactions. (C) 2017 Elsevier B.V. All rights reserved.