Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Using Leaf Extracts of Carissa carandas

摘要

In the present study, silver nanoparticles were synthesized from aqueous silver nitrate solution using leaf extracts of Carissa carandas and screening the activity of the synthesized nanoparticles against different microorganisms. Leaf extract was added to aqueous silver nitrate solution. After few hours colour of the solution changed from yellowish to brown. The confirmation of the nanoparticles was done by UV-visible spectroscopy; SEM and TEM analysis were done to further investigate the shape and size of the nanoparticles. TEM images reveal mat the average size of the nanoparticles was 28 nm. EDAX was used for the identification of multi-elements. The Carissa carandas plant leaf extracts are capable of producing silver nanoparticles extracellularly. The silver nanoparticles were quite stable in solution. The synthesized nanoparticles were screened for antimicrobial activity on five different microorganisms by agar well diffusion method. The nanoparticles have exhibited good antimicrobial activity against the five bacterial stains which were tested viz. Klebsiella pneumoniae, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Out of these, synthesized silver nanoparticles have shown the best inhibition efficiency against Klebsiella pneumoniae.