Four Factor Surface Response Optimization of Ethyl Palmitate from Terminalia cattapa L. Kernel Oil

摘要

Optimization of ethyl palmitate through enzymatic reaction with lipozyme of Terminalia cattapa L. kernel oil and ethanol was carried out. Response surface methodology (RSM) based on a five-level four-factor central composite rotatable design (CCRD) was used to evaluate the interactive effects of synthesis, of reaction time (3-7 h), temperature (35-55 °C), amount of enzyme (0.1-0.3 g) and substrate molar ratio (kernel oil to ethanol, 1:1-1:3) on the percentage yield of ethyl palmitate The optimum conditions obtained in the amount of enzyme 0,19 g, molar ratio of ketapang seed oil:ethanol of 1:2,5 (g/g), a reaction time of 4.68 h and a temperature of 44.82 °C with a predicted yield of 40.031 %. The model recommended to obtain optimal results is a quadratric model with R-squared value of 0.7955.