Interaction of CT-DNA with Ruthenium(II) Metallosurfactant Complexes: Synthesis, CMC Determination, Antitumour and Antimicrobial Activities

摘要

To enhance the application of metalosurfactants in the field of drug delivery, it is essential to acquire the role of surfactants in terms of micellization, hydrophobicity, interaction with nucleic acids, reactive changes with abnormal cells and pathogenic organisms. A new class of two ruthenium(II) metallosurfactant complexes [Ru(DMP)2(CA)Cl](ClO4) (1) and [Ru(DMP)2(CA)2](ClO4)2 (2), where DMP = 2,9-dimethyl[1,10]phenanthroline) and CA = cetyl amine were synthesized and characterized. The critical micelle concentration (CMC) and the thermodynamic parameters of micellization were determined and the variations suggest the expression of hydrophobic interaction in these complexes. The binding affinity of ruthenium(II) metallosurfactant complexes with CT-DNA has been investigated by spectroscopic and viscosity magnitudes. The outcomes expose that the complexes associate with CT-DNA through intercalation mode. Subsequently the complexes were taken for in vitro anticancer and antimicrobial inhibition study against human cervical cancer cell lines (HeLa) and pathogenic microorganisms and found that the complexes exhibited remarkable inhibitory action. The cytotoxic nature of the complexes towards, HeLa cells, was adopted by MTT assay and apoptosis were examined by AO/EB (acridine orange/edudium bromide) and tryphanblue staining methods showing that complexes affected the viability of the cells significantly.