Easy access to efficient magnetically recyclable separation of histidine-tagged proteins using superparamagnetic nickel ferrite nanoparticle clusters

摘要

The simple and efficient separation of histidine-tagged (His-tagged) proteins using nickel ferrite (NiFe2O4) nanoparticle clusters (NPCs) is described in this article. While nanostructured materials containing Ni~(2+) ions for efficient separation were generally synthesized via complex synthetic procedures, the NiFe2O4 nanoparticle clusters used in this work were simply synthesized by a one-pot hydrothermal polyol process. Their large surface area (105.0 m~2 g~(-1)) and pore volume (0.32 cm~3 g~(-1)) provide sufficient sites for the separation of His-tagged proteins. The high magnetic saturation value (41.3 emu g~(-1)) and superparamagnetic property of these nanoparticle clusters lead to a more efficient magnetically recyclable separation of His-tagged proteins. We confirmed that the binding capacity and selective separation ability seen in the first separation were strongly maintained for up to 7 cycles.