Crystal Structure of Hypusine-Containing Translation Factor eIF5A Bound to a Rotated Eukaryotic Ribosome

摘要

Eukaryotic translation initiation factor elF5A promotes protein synthesis by resolving polyproline-induced ribosomal stalling. Here, we report a 3.25-A resolution crystal structure of elF5A bound to the yeast 80S ribosome. The structure reveals a previously unseen conformation of an eIF5A ribosome complex and highlights a possible functional link between conformational changes of the ribosome during protein synthesis and the eIF5A ribosome association. (C) 2016 Published by Elsevier Ltd.