Fabrication of bioactive polycaprolactone/hydroxyapatite scaffolds with final bilayer nano-/micro-fibrous structures for tissue engineering application

摘要

In this study, two techniques, namely electrospinning and needle-punching processes, were used to fabricate bioactive polycaprolactone/hydroxyapatite scaffolds with a final bilayer nano-/micro-fibrous porous structure. A hybrid scaffold was fabricated to combine the beneficial properties of nanofibers and microfibers and to create a three-dimensional porous structure (which is usually very difficult to produce using electrospinning technology only). The first part of this work focused on determining the conditions necessary to fabricate nano- and micro-fibrous components of scaffold layers. A characterization of scaffold components, with respect to their morphology, fiber diameter, pore size, wettability, chemical composition and mechanical properties, was performed. Then, the same process parameters were applied to produce a hybrid bilayer scaffold by electrospinning the nanofibers directly onto the micro-fibrous nonwovens obtained in a traditional mechanical needle-punching process. In the second part, the bioactive character of a hybrid nano-/ micro-fibrous scaffold in simulated body fluid (SBF) was assessed. Spherical calcium phosphate was precipitated onto the nano-/micro-fibrous scaffold surface proving its bioactivity.