Preparation and characterization of poly(triallyl isocyanurate -co-trimethylolpropane triacrylate) monolith and its applications in theseparation of small molecules by liquid chromatography

摘要

A new polymeric monolith was prepared in stainless-steel column and fused-silica capillary, respec-tively, by atom transfer radical polymerization technique. In the polymerization, triallyl isocyanurate(TAIC) was used as the functional monomer; trimethylolpropane triacrylate (TMPTA) as the crosslinkingagent; polyethylene glycol 200 and 1,2-propanediol as the co-porogens; carbon tetrachloride as the ini-tiator and ferrous chloride as the catalyst. The conditions of polymerization were optimized. Morphologyof the prepared poly(TAIC-co-TMPTA) monolith was investigated by scanning electron microscopy; poreproperties were assayed by mercury porosimetry and nitrogen adsorption. The characterization indi-cated that the prepared reversed-phase monolith possessed uniform structure, good permeability andmechanical stability. The column was used as the stationary phase of reversed phase high performanceliquid chromatography (RP-HPLC) and capillary liquid chromatography (CLC) to separate the mixture ofaromatic compounds. The new column performed around 125,000 theoretical plates per meter. The col-umn showed good reproducibility: the relative standard deviation values of the retention factor valuesfor aromatic compounds were less than 1.52% (n = 7, column-to-column).