RAPID DEVELOPMENT AND CHARACTERIZATION OF MICROSATELLITE MARKERS FOR THE SANDHOPPER TRINORCHESTIA LONGIRAMUS JO, 1988 (AMPHIPODA, TALITRIDAE) IN KOREA USING A 454 PYROSEQUENCING APPROACH

摘要

The sandhopper Trinorchestia longiramus Jo, 1988 (Amphipoda, Talitridae) is indigenous to Korea and has great ecological importance as a key species in the food web. To assess the ecological impact of environmental disturbance, we developed microsatellite markers for T. longiramus using 454 pyrosequencing. Thirteen of 35 primer sets producing quality PCR products were polymorphic. In an analysis of 88 individuals from two T. longiramus populations, the mean number of alleles per locus was 8.5, and the mean observed and expected heterozygosities were 0.53 and 0.56, respectively. No linkage disequilibrium was found between any pair of loci. Significant genetic differentiation was detected between the populations. A significant deviation from the Hardy-Weinberg equilibrium was observed at one locus. The cross-amplification test in two sympatric species that are classified in the same family, showed the amplification of two loci. The high degree of polymorphism of these 13 microsatellites will be useful for future conservation genetic studies of this species.