The present invention provides microsatellite marker DNA for discriminating a habitat of Trinorchestia longiramus, in particular, determining whether Trinorchestia longiramus is derived from Geoje area, and a kit and a method for determining a habitat of a Trinorchestia longiramus on the basis of a PCR technique using the same. The kit comprises primer pairs capable of respectively specifically amplifying: 50 to 500 nt of a polynucleotide which is selected from any one polynucleotide selected from the group consisting of SEQ ID NOs: 1 to 7 and includes a continuous (CTT)_(2-15) base sequence; 50 to 500 nt of a polynucleotide which is selected from any one polynucleotide selected from the group consisting of SEQ ID NOs: 8 to 14 and includes a continuous (AAAC)_(3-9) base sequence; and 50 to 500 nt of a polynucleotide which is selected from any one polynucleotide selected from the group consisting of SEQ ID NOs: 15 to 19 and includes a continuous (ACT)_(10-14) base sequence.
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机译:本发明提供了一种微卫星标记DNA,其用于鉴别长鼻Tri的栖息地,特别是确定长鼻nor是否源自巨济地区,以及基于长鼻Tri的PCR技术来确定长鼻Tri的栖息地的试剂盒和方法。相同。该试剂盒包含能够分别特异性扩增:50至500 nt多核苷酸的引物对,该多核苷酸选自选自SEQ ID NOs:1至7的任何一种多核苷酸,并包括连续的(CTT)_(2-15) )基本序列; 50至500nt的多核苷酸,该多核苷酸选自选自SEQ ID NO:8至14的任何一种多核苷酸,并且包括连续的(AAAC)_(3-9)碱基序列; 50至500nt的多核苷酸,该多核苷酸选自选自SEQ ID NO:15至19的任何一种多核苷酸,并且包括连续的(ACT)_(10-14)碱基序列。
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