A novel injectable and in situ crosslinked hydrogel based on hyaluronic acid and α,β-polyaspartylhydrazide

摘要

A new injectable and in situ crosslinked hydrogel, based on a hyaluronic acid (HA) derivative and α,β-polyaspartylhydrazide (PAHy), was produced during the research. This biodegradable and high molecular weight hydrogel can be in situ crosslinked in 15 s by the condensation reaction between aldehyde and amine groups. The HA derivative carrying aldehyde (HAALD) was oxidized from HA by sodium periodate, while the synthesis of hydrogel was performed in a phosphate-buffered saline solution (PBS) using HAALD and PAHy without addition of crosslinker or catalyst. The pH and concentration of the reaction solution, considered as the important factors of the amine-aldehyde reaction, were changed to reveal the crosslinking rule. Thereafter, crosslinked hydrogels were characterized by gelation time, gel content, swelling ratio, and in vitro degradation. Furthermore, the modified polymers were characterized by Fourier transformed infrared (FTIR) spectroscopy to examine their structures. Results from scanning electron microscope (SEM) observations confirmed that a freeze-dried sample revealed a porous hydrogel structure with interconnected pores. The measurement of the cell adhesion confirmed the application potential of HAALD-PAHy hydrogels.