Preparation of Bovine Serum Albumin-Imprinted Calcium Polyacrylate/Alginate Hybrid Microspheres Via Ca2+ Crosslinking

摘要

Macromolecularly imprinted calcium polyacrylate/alginate hybrid polymer microspheres with the imprinting of bovine serum albumin Were prepared with sodium alginate and sodium polyacrylate by using CaCl2, as a gelling agent in inverse-phase suspension. Infrared spectrum and conductance titration demonstrated that hybrid components were produced in these microspheres. Rebinding isotherms of the microspheres proved a greater bovine serum albumin affinity for imprinted microspheres relative to nonimprinted ones. Selectivity tests indicated that calcium polyacrylate/alginate hybrid polymer microspheres exhibited good recognition properties for the template protein with the separation factor over 5.30 comparing to the competitive proteins with a similar M-omega. The influence of cross-linker CaCl2 concentration on the imprinting efficiency (IE) and rebinding capacity,vas researched. It was found that the cross-linking density and imprinting efficiency of the hybrid hydrogel could be improved simultaneously.