Bone cell viability on methacrylic acid grafted and collagen immobilized porous poly(3-hydroxybutrate-co-3-hydroxyvalerate)

摘要

Porous poly(3-hydroxybutrate-co-3-hydroxyvalerate) (PHBV) film was prepared by solute leaching of salt/ PHBV cast film. The surface chemistry of the PHBV membrane was modified by performing graft polymerization of methacrylic acid (MAA) on ozone treated porous PHBV film, followed by immobilization of type I collagen. The surface characteristics of the modified and nonmodified porous films were measured by water contact angle. The rat osteosarcoma cell line UMR-106 osteoblast like cells were used as model cells to evaluate the cell viability on surfaces. The initial cell attachment, growth pattern, and proliferation as measured by MTT assay were used to evaluate the bone cell viability on the modified and nonmodified films. Among the PHBV films studied, the nonmodified porous PHBV and the porous PHBV film type I collagen dip coated showed no significant difference in cell attachment and proliferation, while the porous PHBV membrane that was collagen immobilized after MAA grafting showed considerable activity of osteoblast like cells. (c) 2005 Wiley Periodicals, Inc.