Impact of Dietary Polydextrose Fiber on the Human Gut Metabolome

摘要

The aim. of the present study was to elucidate the impact of polydextrose PDX, an soluble fiber, on the human fecal metabolome by high-resolution nuclear magnetic resonance (NMR) speetroscopyvbased metabolomics in a dietary intervention study (n = 12), Principal component analysis (PCA) revealed a strong effect of PDX consumption on the fecal metabolome, which could be mainly ascribed to the presence of undigested fiber.arid oligosaccharides formed from partial degradation of PDX. Our results demonstrate that NMR-based metabolomics is a useful technique for metabolite profiling of feces and for testing compliance to dietary fiber intake in such trials. In addition, hovel associations between PDX and the levels of the fecal metabolites acetate and propionate could be identified. The establishment of a correlation between the fecal metabolome and levels of Bifidobacterium (R~2 = 0.66) and Bacteroides (ft~2 = 0.46) demonstrates the potential of NMR-based metabolomics to elucidate metabolic activity of bacteria in the gut.