Development and Application of a Quantitative Fluorescence-Based Immunochromatographic Assay for Fumonisin Bi in Maize

摘要

A fluorescence-based immunochromatographic assay (1CA) for fumonisin B, (FB,) that employs conjugates of fluorescent microspheres and monoclonal antibodies (FM—mAbs) as detection reporters is described. The 1CA is based on the competitive reaction between FB1-bovine serum albumin (BSA; test line) and the target FB1 for binding to the FM—mAb conjugates. A limit of detection (LOD) for FB1 of 0.12 ng/mL was obtained, with an analytical working range of 0.2S—2.0 ng/ mL (corresponding to 250—2000 μg/kg in maize flour samples, according to the extraction procedure). The recoveries of the ICA to detect FB[ in maize samples ranged from 91.4 to 118.2%. A quantitative comparison of the fluorescence-based 1CA and HPLC-MS/MS analysis of naturally contaminated maize samples indicated good agreement between the two methods (r~2 = 0.93). By replacing the target of interest, the FM-based ICA can easily be extended to other chemical contaminants and thus represents a versatile strategy for food safety analysis.