Evaluation of Inhibitory Effect of Phlorotannins from Ecklonia cava on Triglyceride Accumulation in Adipocyte

摘要

In the present study, a methanolic extract of Ecklonia cava and its solvent-partitioned fractions were evaluated for their antiadipogenic effect in 3T3-L1 adipocytes. One of them, the n-BuOH fraction, effectively reduced lipid accumulation and glucose consumption. In addition, the presence of the n-BuOH fraction in adipocytes suppressed the regulations of adipogenic transcription factors, PPARy and SREBPlc, and adipogenic specific genes, FABP4, FABP1, FAS, LPL, HSL, and ACSl. Further purification of η-BuOH fractions led to the isolation of six phlorotannins (1—6). The six phlorotannins effectively suppressed triglyceride accumulation. Comparative analysis showed that lipid accumulation in adipocytes was dramatically attenuated in the presence of eckstolonol (4).