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Antioxidant System and Protein Pattern in Peach Fruits at Two Maturation Stages

机译:桃果实两个成熟阶段的抗氧化系统和蛋白质模式

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摘要

Peach fruits were selected to study the protein pattern and antioxidant system as well as oxidative parameters such as superoxide radical and hydrogen peroxide accumulation, at two maturity stages, which were chosen for being suitable for the processing industry and fresh consumption. The proteins phosphoenolpyruvate carboxylase, sucrose synthase, and 1-aminocyclopropane-1-carboxylate oxidase, as well as the antioxidants glutathione synthetase and ascorbate peroxidase, appeared as new in the mature peach fruits. Activities of superoxide dismutase (SOD) and components of the ascorbate-glutathione cycle were also measured to explore their role in the two maturity stages studied. Changes in the SOD isoenzyme pattern and an increase in the activities of ascorbate peroxidase, monodehydroascorbate reductase, and glutathione reductase were observed in mature fruits, revealing an efficient system to cope with the oxidative process accompanying ripening.
机译:选择桃子来研究两个成熟阶段的蛋白质模式和抗氧化系统以及氧化参数(如超氧自由基和过氧化氢的积累),以适合加工工业和新鲜食用。在成熟的桃果实中,磷酸烯醇丙酮酸羧化酶,蔗糖合酶和1-氨基环丙烷-1-羧酸氧化酶以及抗氧化剂谷胱甘肽合成酶和抗坏血酸过氧化物酶作为新蛋白质出现。还测量了超氧化物歧化酶(SOD)的活性和抗坏血酸-谷胱甘肽循环的组成,以探讨它们在所研究的两个成熟阶段中的作用。在成熟果实中观察到SOD同工酶模式的变化以及抗坏血酸过氧化物酶,单脱氢抗坏血酸还原酶和谷胱甘肽还原酶活性的增加,揭示了一种应对成熟过程中氧化过程的有效系统。

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