Multidetermination of Four Nitrofurans in Animal Feeds by a Sensitive and Simple Enzyme-Linked Immunosorbent Assay

摘要

In this study,the polyclonal antibody against furazolidone was produced with furazolidone coupling to protein carriers by a diazotization method and glutaraldehyde reaction,respectively.The antibody obtained showed good specificity toward furazolidone and various cross-reactivity toward nitrofurantoin,nitrofura-zone,and furaltadone.Then,an indirect competitive enzyme-linked immunosorbent assay(ELISA)based on the antibody was first developed for multidetermination of four nitrofurans in animal feeds.The limit of detection(LOD)of the method was 0.2-2.1 ng/g depending on the component.After simple extraction,the fortified swine and broiler chicken feed samples were detected with recovery ranges of 75.9-86.4%.Results obtained from ELISA were confirmed by high-performance liquid chromatography(HPLC)with ultraviolet detection.Analysis of the unknown feed samples indicates that ELISA can be a practical tool for screening of nitrofurans in animal feeds before confirmation by HPLC.