Relative Activity of a Tobacco Hybrid Expressing High Levels of a Tobacco Anionic Peroxidase and Maize Ribosome-lnactivating Protein against Helicoverpa zea and Lasioderma serricorne

摘要

Tobacco(Nicotiana tabacum)plants grown from seed obtained by crossing a tobacco line that expressed an activated maize ribosome-inactivating protein(RIP)with a line that overexpressed tobacco anionic peroxidase were tested for their effects on corn earworm Helicoverpa zea and cigarette beetle Lasioderma serricorne larvae as compared to the wild-type plant cross.Significant feeding reductions were noted for transgenic plants expressing both resistance proteins as compared to wild-type plants for both H.zea and L.serricorne.Significant increases in mortality were also noted for those insects fed on the transgenic cross as compared to wild-type plants in some cases.Levels of both peroxidase and maize RIP were significantly higher in transgenic as compared to wild-type plants(which did not produce maize RIP).The degree of feeding was significantly negatively correlated with the level of RIP or peroxidase individually.