Liquid Chromatography Coupled to Isotope Ratio Mass Spectrometry:A New Perspective on Honey Adulteration etection

摘要

A new procedure to determine individual sugar(sucrose,glucose,and fructose)~(13)C isotope ratios,using liquid chromatography-isotope ratio mass spectrometry(HPLC-IRMS),has been developed to improve isotopic methods devoted to the study of honey authenticity.For this purpose 79 commercial honey samples from various origins were analyzed.Values of delta~(13)C_(honey)ranged from-14.2 to-27.2%,and delta ~(13)C_(protein)ranged from-23.6 to-26.9%.A very strong correlation is observed between the individual sugar ~(13)C ratios,which are altered in the event of sugar addition,even at low levels.The use of DELTA delta~(13)C [fruct-glu],DELTA delta~(13)C [fruct-suc],and DELTA delta~(13)C [gluc-suc] systematic differences as an authenticity criterion permits the sugar addition [C_3,beet sugar;or C_4,cane sugar,cane syrup,isoglucose syrup,and high-fructose corn syrup(HFCS)] to be reliably detected(DL = 1-10%).The new procedure has advantages over existing methods in terms of analysis time and sensitivity.In addition,it is the first isotopic method developed that allows beet sugar addition detection.