Authenticity assessment of gamma- and delta-decalactone from Prunus fruits by gas chromatography combustion/pyrolys is isotope ratio mass spectrometry (GC-C/P-IRMS)

摘要

Authenticity assessment of gamma-decalactone (1) and delta-decalactone (2) from peach (Prunus persica var. persica), apricot (Prunus armeniaca), and nectarine (Prunus persica var. nectarina) was performed using gas chromatography-isotope ratio mass spectrometry (GC-IRMS) in the combustion (C) and pyrolysis (P) mode. In addition, commercially available synthetic (nature-identical) 1 and 2 as well as biotechnologically produced samples (declared to be "natural") were characterized by their delta(2)H(V-SMOW) and delta(13)C(V-PDB) values. For the Prunus fruits under study, rather narrow ranges of delta(13)C(V-PDB) and delta(2)H(V-SMOW) data of 1, varying from - 34.6 parts per thousand to - 38.4 parts per thousand and -160 parts per thousand to -206 parts per thousand, respectively, were obtained. Synthetic references of 1 showed delta(13)C(V-PDB) and delta(2)H(V-SMOW) data ranging from -27.4 parts per thousand to -28.3 parts per thousand and -151 parts per thousand to -184 parts per thousand, respectively. Samples of 1 declared to be "natural" exhibited ranges from -28.1 parts per thousand to -29.2 parts per thousand and - 192 parts per thousand to -286 parts per thousand for delta(13)C(V-PDB) and delta(2)H(V-SMOW), respectively. For 2 from peach, apricot, and nectarine, delta(13)C(V-PDB) values ranging from -34.0 parts per thousand to -37.9 parts per thousand were determined; the delta(2)H(V-SMOW) values ranged from -171 parts per thousand to -228 parts per thousand. The delta(13)C(V-PDB) and delta(2)H(V-SMOW) data for synthetic 2 were -28.2 parts per thousand and -171 parts per thousand, respectively, that is, similar to those of 2 from "natural" origin, ranging from -27.7 parts per thousand to -30.1 parts per thousand and -185 parts per thousand to -230 parts per thousand for delta(13)C(V-PDB) and delta(2)H(V-SMOW), respectively. GC-C/P-IRMS allowed clear-cut analytical differentiation of the synthetic and "ex-plant" origin of 1 and 2, whereas narrow ranges of delta(13)C(V-PDB) and delta(2)H(V-SMOW) data were found for samples of synthetic and "natural" origin.