Evaluation of Nonstarch Polysaccharides and Oligosaccharide Content of Different Soybean Varieties(Glycine max)by Near-Infrared Spectroscopy and Proteomics

摘要

A total of 832 samples of soybeans were screened by near-infrared(NIR)reflectance spectroscopy,to identify soybean samples with a lower content of oligosaccharides and nonstarch polysaccharides(NSP).Of these,38 samples were identified on the basis of variation in protein content and agronomic value and submitted to high-resolution NIR spectroscopy.On the basis of the NIR data,12 samples were further selected for chromatographic characterization of carbohydrate composition(mono-,di-,and oligosaccharides and NSP).Their soluble proteins were separated by two-dimensional gel electrophoresis(2DE).Using partial least-squares regression(PLSR),it was possible to predict the content of total NSP from the high-resolution NIR spectra,suggesting that NIR is a suitable and rapid nondestructive method to determine carbohydrate composition in soybeans.The 2DE analyses showed varying intensities of several proteins,including the glycinin G1 precursor.PLSR analysis showed a negative correlation between this protein and insoluble NSP and total uronic acid(UA).