Re: Analysis of the outcome of intracytoplasmic sperm injection using fresh or frozen sperm

摘要

Objectives: To compare the outcome of first-attempt intracytoplasmic sperm injection (ICSI) ICSI-embryo transfer (ET) cycles using frozen-thawed testicular sperm (FTTS), fresh testicular sperm (FTS), frozen-thawed epididymal sperm (FTES) and fresh epididymal sperm (FES) so as to determine which of these has the most successful ICSI outcome with respect to fertilization rate (FR), pregnancy rate (PR) and birth rate. To assess the outcomes according to the underlying aetiology of azoospermia. Patients and Methods: The records of 493 patients undergoing first-attempt ICSI between 1993 and 2008 were reviewed retrospectively. FTS was used in 112 cycles, FTTS in 43 cycles, FES in 279 cycles, and FTES in 59 cycles. Within each group, the aetiology of the azoospermia was recorded according to history, clinical examination and histological analysis (n = 316). The FR, clinical PR and delivery rate were calculated for each group with respect to the type of sperm retrieval used.