ACE gene insertion/deletion polymorphism seminal associations in infertile men

摘要

Purpose: We assessed seminal associations of the ACE (*) gene insertion/deletion polymorphism in infertile men. Materials and Methods: A total of 405 men were investigated, divided into healthy fertile men, and those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. They underwent semen analysis, and assessment of sperm acrosin activity, hypo-osmotic swelling, seminal 8-iso-prostaglandin-F 2α, total antioxidant capacity, α-glucosidase and ACE gene polymorphisms. Result: The ACE (*) insertion/insertion genotype was noted in 182 men, including 76.5% of healthy fertile men, and 47.4%, 39.8% and 17.6% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. The ACE (*) insertion/deletion genotype was noted in 133 men, including 13.7% of healthy fertile men, and 42.3%, 27.5% and 47.2% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. The ACE (*) deletion/deletion genotype was identified in 90 men, including 9.8% of healthy fertile men, 10.3%, 32.70% and 35.2% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. Men with the ACE (*) deletion/deletion and insertion/deletion genotypes showed a significant decrease in sperm count, motility, linear velocity and normal forms, acrosin activity index, hypo-osmotic swelling test and seminal α-glucosidase, and significantly increased seminal 8-iso-prostaglandin- F 2α than those with the ACE (*) insertion/insertion genotype. Conclusions: ACE gene deletion polymorphism is associated with abnormal seminal variables, such that carriers of the ACE (*) deletion/deletion genotype have higher seminal oxidative stress.