ACE基因插入/缺失多态性与228例原发性高血压的相关性研究

摘要

Objective To investigate the relationship between angiotensin converting enzym e(ACE) gene insertion/deletion polym orphism and essential hypertension (EH) and different hypertension grades in Qingdao area.Methods 228 essential hypertension sufferers and 119 peo-ple with normal blood pressure in Qingdao area were chosen to detect blood pressure,blood lipid ,blood glucose and other clinical and bio-chemical indexe sinboth groups,and the blood pressure was classified into three grades.The polymerase chainreaction(PCR)techniquewas adopted to determ ine ACE gene I/D polym orphism .Results T he difference of genotype frequency of ACE gene type was not of statistical significance in EHgroup and normal group (P>0 .05 ).T he difference of allele frequency was of statistical significance in EHgroup and nor-m al group (P<0 .05 ).H ierarchy was further made on the basis of gender.T he difference of genotype and allele distribution frequency was of statistical significance between males in EHgroup and normal group (P<0 .05 ),but it was not of statistical significance between females in EHgroup and normal group (P>0 .05 ).A ccording to trend test results,the Dallele is positively correlated with blood pressure grades in EHgroup .Conclusion T he Dallele of ACE is probably involved in the pathogenesis of EHin Qingdao area,especially in males,and also relat-ed to blood pressure grades.%目的：探讨血管紧张素转化酶(ACE)基因插入/缺失(I/D)多态性与青岛地区原发性高血压(EH)的发生以及高血压分级间的相关性。方法选择青岛地区EH患者228例和119例正常对照，测定两组血压、血脂、血糖等临床及生化指标，并对高血压进行分级。采用聚合酶链反应技术(PCR)，检测ACE基因型。结果 EH组与对照组相比，两组间基因型频率比较差异无统计学意义(P＞0.05)，等位基因频率比较差异有统计学意义(P＜0.05)。进一步以性别进行分层，男性EH组与男性对照组相比，基因型及等位基因分布频率差异均有统计学意义(P＜0.05)；而女性EH组与女性对照组相比，基因型及等位基因分布频率差异均无统计学意义(P＞0.05)。结合趋势检验，EH组中等位基因D与血压的分级呈正相关(P＜0.05)。结论 ACE基因等位基因D与EH的发生相关，尤其与男性EH相关，并与血压的分级相关联。