Development of Activity-Based Probes for Imaging Human alpha-L-Fucosidases in Cells

摘要

We have established a concise synthetic route relying on a key base-promoted epimerization step to synthesize two series of activity-based probes carrying a BODIPY fluorophore for alpha-L-fucosidase. The resulting probes were evaluated for labeling performance. The one utilizing an o-fluoromethylphenol derivative as the latent trapping unit was successfully applied for the first time to visualize and locate lysosomal alpha-L-facosidase activity in human cells.