Collective diffusion coefficient of proteins with hydrodynamic, electrostatic, and adhesive interactions

摘要

A theory is presented for lambda(C), the coefficient of the first-order correction in the density of the collective diffusion coefficient, for protein spheres interacting by electrostatic and adhesive forces. An extensive numerical analysis of the Stokesian hydrodynamics of two moving spheres is given so as to gauge the precise impact of lubrication forces. An effective stickiness is introduced and a simple formula for lambda(C) in terms of this variable is put forward. A precise though more elaborate approximation for lambda(C) is also developed. These and numerically exact expressions for lambda(C) are compared with experimental data on lysozyme at pH 4.5 and a range of ionic strengths between 0.05M and 2M. (c) 2007 American Institute of Physics.