Microarray-Based Genomic Selection for High-Throughput Resequencing

摘要

A major bottleneck in DNA sequencing is in isolating the target DNA to be sequenced PCR, the most common method of sample preparation, is not only difficult to scale to large genomic regions, but also labor-intensive and subject to failure or artifacts. David Okou and colleagues developed Microarray-based Genomic Selection (MGS), a method capable of isolating user-defined, unique genomic sequences from complex eukaryotic genomes. The MGS protocol consists of the following steps [Figure 1).