Spectrally encoded flow cytometry (SEFC) is a promising technique for imaging blood in the microcirculation. Yet, the dependency of one of the axes of the image on time prevents effective quantification of essential clinical parameters. Here, we address this challenge by splitting the optical path in an SEFC system into two parallel imaging lines, followed by straightforward data analysis for recovering the flow speed from the multiplexed data. The method is demonstrated by measuring the flow velocity of latex beads and blood cells in vitro. The system allows real-time velocity measurements of up to 11.7 mm/s at high spatial resolution, and could be integrated into existing SEFC systems for effectively measuring blood parameters in small capillary vessels.
展开▼
机译：光谱编码流式细胞仪（SEFC）是一种用于微循环血液成像的有前途的技术。然而，图像的轴之一对时间的依赖性阻止了基本临床参数的有效量化。在这里，我们通过将SEFC系统中的光路分成两条平行的成像线，然后进行简单的数据分析以从多路复用数据中恢复流速来应对这一挑战。通过体外测量乳胶珠和血细胞的流速证明了该方法。该系统可以在高空间分辨率下进行高达11.7 mm / s的实时速度测量，并且可以集成到现有的SEFC系统中，以有效地测量小型毛细血管中的血液参数。
展开▼
