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Efficient optimization of super-oscillatory lens and transfer function analysis in confocal scanning microscopy

机译:共聚焦扫描显微镜中超振荡透镜的高效优化和传递函数分析

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摘要

Super-oscillatory lens (SOL) provides a promising way to achieve subwavelength focusing in the regime of far-field optics and realize super-resolution imaging in confocal scanning microscopy (CSM). Both binary amplitude and phase SOLs are designed with an efficient optimization method using genetic algorithm and fast Hankel transform algorithm either in oil immersion medium or in air. A much brighter hotspot is readily focused by a phase SOL compared with the amplitude counterpart, e.g., 5.8 times as bright as the latter. To fundamentally interpret the super-resolution imaging mechanism by SOL in CSM, transfer function analysis is conducted compared with basic confocal imaging. The coherent transfer function (CTF) is derived and numerically calculated. The extension of the cutoff frequency and the remarkable enhancement of the magnitude of CTF in the high frequency passband account for super-resolution imaging by SOL in CSM; however, the limited extension of the frequency domain also implies that the attainable resolution is physically limited.
机译:超级振荡透镜(SOL)提供了一种有前途的方法,可在远场光学领域实现亚波长聚焦,并在共聚焦扫描显微镜(CSM)中实现超分辨率成像。在油浸介质或空气中,使用遗传算法和快速汉克尔变换算法,通过有效的优化方法设计了二进制幅度和相位SOL。相较于幅度对应物,相位SOL更容易聚焦更亮的热点,例如,是后者的5.8倍。为了从根本上解释SOL在CSM中的超分辨率成像机理,将传递函数分析与基本共聚焦成像进行了比较。相干传递函数(CTF)导出并进行数值计算。高频通带中截止频率的扩展和CTF幅度的显着提高说明了CSM中SOL的超分辨率成像。但是,频域的有限扩展也意味着可获得的分辨率在物理上受到限制。

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