Dynamics in cytoplasm, nucleus, and lipid droplet of a live CHO cell: Time-resolved confocal microscopy

摘要

Different regions of a single live Chinese hamster ovary (CHO) cell are probed by time-resolved confocal microscopy. We used coumarin 153 (C153) as a probe. The dye localizes in the cytoplasm, nucleus, and lipid droplets, as is clearly revealed by the image. The fluorescence correlation spectroscopy (FCS) data shows that the microviscosity of lipid droplets is ～34 ± 3 cP. The microviscosities of nucleus and cytoplasm are found to be 13 ± 1 and 14.5 ± 1 cP, respectively. The average solvation time (〈τ_s〉) in the lipid droplets (3600 ± 50 ps) is slower than that in the nucleus (〈τ_s〉 = 750 ± 50 ps) and cytoplasm (〈τ_s〉 = 1100 ± 50 ps). From the position of emission maxima of C153, the polarity of the nucleus is estimated to be similar to that of a mixture containing 26% DMSO in triacetin (η ～ 11.2 cP, ε ～ 26.2). The cytoplasm resembles a mixture of 18% DMSO in triacetin (η ～ 12.6 cP, ε ～ 21.9). The polarity of lipid droplets is less than that of pure triacetin (η ～ 21.7 cP, ε ～ 7.11).