BIOMOLECULE TRANSDUCTION MOTIF SIM-2-BTM DELIVERING BIOLOGICAL RESPONSE MODIFIER TO CYTOPLASM, ORGANELLES OR NUCLEUS AND THE USE THEREOF FOR DELIVERING BIOLOGICAL RESPONSE MODIFIER TO CYTOPLASM, ORGANELLES OR NUCLEUS OF PROKARYOTIC AND EUKARYOTIC CELLS

摘要

PURPOSE: A biomolecule transduction motif Sim-2-BTM and the use thereof are provided. The motif delivers a biological response modifier to cytoplasm, organelles or nucleus of prokaryotic and eukaryotic cells. CONSTITUTION: A peptide comprises the amino acid sequence of SEQ ID NO:1 or its active fragment, for transducing a biological response modifier into prokaryotic or eukaryotic cells, wherein at least any one of Arginine, Lysine and Alanine is substituted with a structurally and functionally similar amino acid; the biologically active functional regulatory molecule is any one of selected from protein, DNA, RNA, carbohydrate, lipid and chemical compound; and the peptide or its active fragment is transduced into the cells of prokaryotes or eukaryotes through administration routs comprising intramuscular, intraperitoneal, intravein, oral, nasal, subcutaneous, intradermal, mucosal and inhaling routes. A recombinant expression vector comprises DNAs encoding the peptide or its active fragment, wherein DNAs encoding one or more homologous or heterologous protein as a biologically active functional regulatory molecule and operably linked expression regulatory sequence. A biomolecule transduction complex comprises (i) the peptide or its active fragment; and (ii) one or more biologically active functional regulatory molecule selected from protein, DNA, RNA, carbohydrate, lipid and chemical compound, wherein the molecule of (ii) is fused or bound to the peptide or its fragment of (i) chemically and physically.