Effects of different concentrations of oxygen - ozone on rats' astrocytes in vitro.

摘要

Although the widespread use of the oxygen-ozone in pain management, there is currently no consensus on its mechanisms of action and nearly no report for its action on nervous cells. Accordingly, the present study was designed to assess the effects of oxygen-ozone on astrocytes. Astrocytes were cultured in vitro through methods of trypsinization, different-speed cultivation and passaging to purify, then seeded into 24 well plates and divided to one of four groups (n=7) to receive the following treatments: respectively added 400mul complete medium (CM) after effects of 20mug/ml oxygen-ozone (Group O-20), 40mug/ml oxygen-ozone (Group O-40), 60mug/ml oxygen-ozone (Group O-60); without intervention (Group C). After incubation of 2h or 4h, cell morphology was observed and endocellular superoxide dismutase (SOD), endocellular malondialdehyde (MDA), lactate dehydrogenase (LDH) leaking ratio, and dead cells' percentage were detected. The results showed cell damage in Group O-60. As compared with Group C, endocellular SOD increased in all groups, MDA at 2h increased in Groups O-40 and O-60 and MDA at 4h decreased in Groups O-20 and O-40; LDH leaking ratio at 2h in Group O-20 and those at 2 and 4h in Group O-40 decreased, while LDH leaking ratio at 4h increased and dead cells' percentage in Group O-60 increased. We conclude that in short time (2 and 4h), oxygen-ozone of 60mug/ml showed a damaging role on astrocytes in vitro, while oxygen-ozone of 20 and 40mug/ml did not show damaging role obviously.