Protein folding following synthesis in vitro and in vivo: Association of newly synthesized protein with 50S subunit of E-coli ribosome

摘要

In the accompanying paper, it was shown that a protein, while reverting to native form from the unfolded state in vitro with the help of bacterial 70S ribosome, split the latter into its subunits (50S and 30S) and remains associated with the 50S subunit. Here, we follow the fate of nascent proteins both in case of in vivo and in vitro translation system. The newly synthesised protein was found to associate with the 50S subunit in both the cases. (C) 2007 Elsevier Inc. All rights reserved.