Ameliorative effect of acetyl-L-carnitine and/or nifedipine against selenite-induced cataractogenesis in young albino rats

摘要

Free radical toxicity and calcium ion overload have been identified as the major two players in the causation of cataract. The current study was carried out to investigate the anti-cataractogenic effect of single and combined treatment with acetyl-L-carnitine and nifedipine in sodium selenite-induced cataract. Rat pups were divided into 5 groups; 1st group received intraperitoneal injection (i.p.) of saline and served as normal control, 2nd group received single subcutaneous injection of sodium selenite 30 nmol/g body weight on plO (postpartum day 10), 3rd and 4th groups received either acetyl-L-carnitine (200 mg/kg, i.p.) or nifedipine (0.1 mg/kg, i.p.) on p9, respectively, before the administration of sodium selenite, and the treatment continued till pl4. Last group received the combined treatments of acetyl-L-carnitine and nifedipine in the same regimen. All animals were examined using a slit lamp and retroillumination then sacrificed on p30. Lenses were removed and processed for biochemical analyses, histopathological and electron microscopic examination. Selenite-treated groups showed significantly (P < 0.05) lower values of redox system components (glutathione and glutathione reductase activity) and anti-oxidant enzymes' activities (superoxide dismutase and catalase) along with increased lipid peroxidation that was accompanied by 100% opacified crystalline lenses (mature cataract) with abnormal structure as detected by electron microscopy. It is concluded that acetyl-L-carnitine or nifedipine was able to partially protect against selenite-induced abnormalities. While, combined treatment with acetyl-L-carnitine and nifedipine was superior to individual treatments in slowing down the development of cataract by restoring the anti-oxidant defense and mitigating lipid peroxidation in the lens and hence represents an attractive anti-cataractogenic remedy.