Functionalization of (CdSe)ZnS nanoparticles in reverse micelles of aerosol ot

摘要

A method is optimized for in situ functionalization and activation of (CdSe)ZnS nanoparticles in reverse micelles of Aerosol OT, as well as their conjugation with antibodies. The efficiency of particle func- tionalization depends on the method of micellar solution formation. Fluorescence remains practically unchanged, provided that NH _2 groups of polymers bonded to the nanoparticles are activated by glutaric alde- hyde. All operations of antibody conjugation should be carried out in reverse micelles for the maximum pres- ervation of the fluorescence properties of the particles. Nanoparticles are isolated from reverse micelles using a mixture of acetone with a solution composed of 50 mM NaHCO _3 and 200 mM NaCl. The optimum medium for dissolving the precipitate of the particles consists of NaHCO _3 (50 mM), bovine serum albumin (0.1%), and Twin 85 (0.05%). The modified particles were subjected to photoactivation in an aqueous medium at room temperature and daylight.