Simultaneous Determination of 13 Nucleosides and Nucleobases in Ganoderma lucidum and Related Species by HPLC-DAD

摘要

A simple, sensitive and reliable HPLC-DAD method was performed for the simultaneous determination of 13 nucleosides and nucleobases including cytosine, uracil, cytidine, hypoxanthine, uridine, thymine, 2'-deoxyuridine, adenine, inosine, guanosine, thymidine, adenosine and 2'-deoxyadenosine in Ganoderma lucidum and its related species. The analysis was conducted on a phenomenex C_(18) column (250 mm x 4.6 mm i.d., 5 mm) eluting with a gradient of acetonitrile in water at a flow rate of 0.6 mL/min and the detection wavelength was set as 260 nm. The optimized method attained good linear relation (r > 0.9996 for 13 analytes), satisfactory precision (RSD < 1.52 % ) and good recovery (from 98.10 to 101.27 %). The validated method was successfully applied to the simultaneous determination of 13 analytes in 42 samples from Ganoderma lucidum and its related species collected from different areas, which could be beneficial to control their quality.