Purification of Total Polyphenols from Porphyra yezoensis

摘要

Total polyphenols extracts from Porphyra yezoensis were pretreated in order to partially remove proteins and polysaccharides before purification by macroporous resins. The most appropriate resin was selected among 10 kinds of macroporous resins. Adsorption and desorption kinetics on AB-8 resins were determined by static adsorption and desorption experiments. The effect of polyphenols initial concentration, adsorption flow velocity, desorption flow velocity and the desorption solution concentration were investigated by using dynamic adsorption and desorption experiment. Polyphenols from Porphyra yezoensis was analyzed by HPLC-MS/MS. The results showed that AB-8 resin was most appropriate to purify total polyphenols, the adsorption capacity reached equilibrium after 4 h. the desorption capacity reached equilibrium after 2 h. The flow rate of absorption or desorption was 1 mL/min, the polyphenols solution concentration was 1 mg/mL. The elution solvent was 90 mL 60 % ethanol. The purity and recovery rate were 81.35 and 72.26 %, respectively. Seven kinds of polyphenols were separated and analyzed by HPLC-MS/MS.