Simultaneous Determination of Five Compounds by HPLC and Its Application to the Suitable Harvest Time for Medicinal Plant Curcuma wenyujin

摘要

The methods for simultaneous determination of five compounds including β-elemene, curcumine, curcumol, curdiohe and germacrone, were developed using a reversed-phase C_(18) column. All calibration curves were linear (r > 0.9993) over the range of tested concentration. This method showed good repeatability for the quantification of these five compounds with intra-day and inter-day variations of less than 1.42 and 1.76 %, respectively. Furthermore, the validated method was applied to determine four active components in rhizome extract from different developmental stages of Curcuma wenyujin. The results showed the suitable harvest time can be suggested before and after January, which is helpful to control their quality.