Synthesis, Characterization and Electrochemical Studies on the Interaction Mechanism of Ternary Cu(II) Complex with DNA

摘要

A new ternary complex of Cu(II) with Schiff base 2-hydroxy-1-naphthaldehyde-glycine (KHL) and 8-hydroxyquinoline (OHQ) was synthesized and characterized by elemental analysis, IR, TG-DTG and molar conductance. The composition of the complex was confirmed to be [Cu(L)(OHQ)] · 2H2O. The fundamental electrochemical characteristics of the Cu(II) complex have been studied and me interaction of the copper complex and DNA was also studied by cyclic voltammetry and fluorescence spectra. The results of the cyclic voltammetry showed that the Cu(II) complex had an oxidation peak wim the oxidation potential at -0.394 V. After the DNA was added, the peak current declined with the peak potential shifting positively. This suggested mat the complex combined with DNA in the form of intercalative binding, which was also proved by the fluorescence analysis. The binding ratio between me Cu(II) ternary complex and DNA was calculated to be 1:1 and the binding constant was 4.6 x 10~2 L mol~(-1).