Chemiluminescence determination of tetracyclines using Fenton system in the presence europium(III) ions

摘要

A new simple chemiluminescent method for the determination of chlortetracycline (Chlor-TC), oxyte-tracycline (Oxy-TC) and doxycycline (Doxy-TC) is described. This method is based on the europium(III) emission as a result of the energy transfer process from the excited product of the tetracyclines oxidation to the uncomplexed Eu(III). Under the optimum conditions, calibration graphs were obtained for 4 x 10~(-7) to 2 x 10~(-5) mol L~(-1) of Chlor-TC; 2 x 10~(-7) to 2 x 10~(-5) mol L~(-1) of Oxy-TC and 1 x 10~(-7) to 3 x 10~(-5) mol L~(-1) of Doxy-TC. The method was successfully applied to the determination of these drugs in pharmaceutical and veterinary formulation and honey.