Identification of Aspartic and Isoaspartic Acid Residues in Amyloid beta Peptides, Including A(beta)1-42, Using Electron-Ion Reactions

摘要

Amyloid beta peptides are the major components of the vascular and plaque amyloid filaments in individuals with Alzheimer's disease (AD). Although it is still unclear what initiates the disease, isomerization of aspartic acid residues in A(beta) peptides is directly related to the pathology of AD. The detection of isomerization products is analytically challenging, due to their similar chemical properties and identical molecular mass. Different methods have been applied to differentiate and quantify the isomers, including immunology, chromatography, and mass spectrometry. Typically, those methods require comparative analysis with the standard peptides and involve many sample preparation steps. To understand the role of A(beta) isomer-ization in AD progression, a fast, simple, accurate, and reproducible method is necessary. In this work, electron capture dissociation (ECD) Fourier-transform ion cyclotron resonance mass spectrometry (FTICR MS) was applied to detect isomerization in A(beta) peptides. ECD generated diagnostic fragment ions for the two isomers of A(beta)17-28, [M + 2H - 60]~(+centre dot) and z_(6)~(centre dot) - 44 when aspartic acid was present and z_(6)~(centre dot) - 57 when isoaspartic acid was present. Additionally, the z_(n) - 57 diagnostic ion was also observed in the electron ionization dissociation (EID) spectra of the modified A(beta)17-28 fragment. ECD was further applied toward A(beta)1-40 and A(beta)1-42. The diagnostic ion c_(6) + 57 was observed in the ECD spectra of the A(beta)1-42 peptide, demonstrating isomer-ization at residue 7. In conclusion, both ECD and EID can clearly determine the presence and the position of isoaspartic acid residues in amyloid beta peptides. The next step, therefore, is to apply this method to analyze samples of Alzheimer's patients and healthy individuals in order to generate a better understanding of the disease.