BSI to study free-solution binding without labeling

摘要

For binding assays designed to study how biomolecules interact, the less sample required and the lower the detection limits, the better. Darryl Bornhop and colleagues at Vanderbilt University and the Technical University of Denmark have used back-scattering interferometry (BSI) to quanti-tate molecular binding at a concentration of 25 pM with an effective detection volume of ~350pL.