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Method for the preparation of a labelled virus without the inactivation of viral binding sites and method of assay utilizing said labelled virus
Method for the preparation of a labelled virus without the inactivation of viral binding sites and method of assay utilizing said labelled virus
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机译:没有灭活病毒结合位点的标记病毒的制备方法和利用所述标记病毒的测定方法
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摘要
A method for the preparation of a labelled target-specific virus where the binding sites on the virus are not inactivated by the label material. And a three-step process for detection of a targeted microorganism, the first step comprising contacting of labelled target- specific virus with a sample suspected of containing a target microorganism, the second step comprising the capture of the labelled virus/microorganism complexes onto a solid matrix, and the third step comprising the detection of said complexes on the solid matrix. The process is highly sensitive and can be used for rapid detection of target microorganisms in clinical, food, pharmaceutical, cosmetic and environmental samples. The invention further concerns a device and a kit based on the inventive process.
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