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Isotope edited internal standard method for quantitative surface-enhanced Raman spectroscopy

机译:同位素编辑的内标法用于定量表面增强拉曼光谱

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摘要

A new isotope edited internal standard (IEIS) method for quantitative surface-enhanced Raman spectroscopy (SERS) is demonstrated using rhodamine 6G (R6G-d(0)) and rhodamine 6G (R6G-d(4)) edited with deuterium. The reproducibility and accuracy of the IEIS method is investigated both under optical resonance (SERRS) and non-resonance (SERS) conditions. A batch-to-batch concentration measurement reproducibility of better than 3% is demonstrated over a concentration range of 200 pM-2 μ M with up to a factor of 3 difference between the concentration of the analyte and its IEIS. The superior performance of the IEIS method is further illustrated by comparing results obtained using absolute SERS/SERRS intensity calibration (with no internal standard) or using adenine (rather than R6G-d4) as an internal standard for R6G concentration quantization. Potential biomedical gene expression and comparative proteomic applications of the IEIS method are discussed.
机译:使用若丹明编辑的若丹明6G(R6G-d(0))和若丹明6G(R6G-d(4))演示了一种用于定量表面增强拉曼光谱(SERS)的新的同位素编辑内标(IEIS)方法。在光学共振(SERRS)和非共振(SERS)条件下,均研究了IEIS方法的重现性和准确性。在200 pM-2μM的浓度范围内,证明了批次之间的浓度测量重现性优于3%,分析物与其IEIS的浓度之差最高可达3倍。通过比较使用绝对SERS / SERRS强度校准(无内标)或使用腺嘌呤(而不是R6G-d4)作为内标进行R6G浓度定量所获得的结果,进一步说明了IEIS方法的优越性能。讨论了潜在的生物医学基因表达和IEIS方法的比较蛋白质组学应用。

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