Phosphonated Near-Infrared Fluorophores for Biomedical Imaging oi Bone

摘要

The conventional method for creating targeted contrast agents is to conjugate separate targeting and fluoro-phore domains. A new strategy is based on the incorporation of targeting moieties into the non-delocalized structure of pen-tamethine and heptamethine indocyanines. Using the known affinity of phosphonates for bone minerals in a model system, two families of bifunctional molecules that target bone without requiring a traditional bisphosphonate are synthesized. With peak fluorescence emissions at approximately 700 or 800 nm, these molecules can be used for fluorescence-assisted resection and exploration (FLARE) dual-channel imaging. Longitudinal FLARE studies in mice demonstrate that phosphonated near-infrared fluorophores remain stable in bone for over five weeks, and histological analysis confirms their incorporation into the bone matrix. Taken together, a new strategy for creating ultra-compact, targeted near-infrared fluorophores for various bioimaging applications is described.