High-Resolution Studies of Uniformly ~(13)C,~(15)N-Labeled RNA by Solid-State NMR Spectroscopy

摘要

Solid-state NMR spectroscopy with magic angle spinning (MAS NMR) is an advanced noninvasive technique to study the structure and dynamics of biologic macromolecules. MAS NMR experiments can be performed in frozen solution, in membranes, and in microcrystalline or freeze-dried proteins. These studies yield information on internuclear distances, torsion angles, molecular orientation, and functional dynamics. MAS NMR spectroscopy provides a unique opportunity to study macromolecules in their own natural environment, in vitro and in vivo, be it a single purified protein, large multiprotein complexes, molecular fibrils, cell organelles, the entire cell or tissue, or the whole organism. In addition, solid-state NMR spectroscopy emerges as a powerful tool for the time-resolved study of macromolecular folding and catalysis. By varying the temperature of the frozen sample, structural and chemical transitions can be selectively trapped or monitored in real time.