PELDOR Spectroscopy Distance Fingerprinting of the Octameric Outer-Membrane Protein Wza from Escherichia coli.

摘要

Membrane proteins account for over one-fifth of the encoded proteins, but their crystallization is challenging, particularly for multiprotein complexes such as the polysaccharide export system of Escherichia coli. One major component of this system is the translocation channel Wza, an octameric outer-membrane protein of 320 kDa whose closed-state crystal structure has been recently determined.