Surface structures of adsorption layers of sodium hyaluronate and bovine serum albumin complexes on poly(gamma-methyl-L-glutamate)film and their surface properties

摘要

Two-dimensional structures and characteristics of the complexes between sodium hyaluronate(NaHA)and bovine serum albumin(BSA)were studied by using a quartz crystal microbalance method and an atomic force microscope(AFM).NaHA did not adsorb on poly(gamma-methyl-L-glutamate)(PMLG)film.On the other hand,the complexes adsorbed on it and the adsorption behaviors were found to be Langmuir types.With increasing weight ratio of BSA to NaHA,W_(BSA),the adsorption constants K decreased and the saturated adsorption masses GAMMA~(infinity)increased.The adsorbed complexes were spherical particles and at saturated adsorption states they covered compactly on the PMLG film.The mean diameters d_(AFM)estimated from the topographic images decreased from 70 to 54 nm with increasing W_(BSA).The adhesion force F_(ad)and the frictional force F_f between the complex layers and the AFM tip were obtained by using the contact mode of the AFM.With increasing W_(BSA),the values of F_(ad)decreased and the values of F_f increased.Compared with the frictional coefficient of the NaHA adsorption layer on the BSA mono-layer,the values for the NaHA-BSA complex layer were found to be much higher.